Sök i kursutbudet

Använda sökfunktionen för att hitta i Chalmers utbildningsutbud, både vad gäller kurser och program. När det finns en kurshemsida visas en hus-symbol som leder till denna sida. Tänk på att välja det läsår du vill se information om.
Sök program och utbildningsplaner


Institutionernas kurser för doktorander

​​​​​​​​​​​​​​​​​​​​

Kursplan för

Läsår
KBB100 - Biochemical toxicology A
 
Kursplanen fastställd 2012-02-21 av programansvarig (eller motsvarande)
Ägare: MPBIO
7,5 Poäng
Betygskala: TH - Fem, Fyra, Tre, Underkänt
Utbildningsnivå: Avancerad nivå
Huvudområde: Bioteknik
Institution: 0240 - ZOOLOGISKA INSTITUTIONEN GU


Undervisningsspråk: Engelska
Sökbar för utbytesstudenter
Blockschema: B

Modul   Poängfördelning   Tentamensdatum
Lp1 Lp2 Lp3 Lp4 Sommarkurs Ej Lp
0107 Tentamen 7,5 hp Betygskala: TH   7,5 hp   26 Okt 2012 em V

I program

MPBIO BIOTECHNOLOGY, MSC PROGR, Årskurs 1 (obligatoriskt valbar)
MPBIO BIOTECHNOLOGY, MSC PROGR, Årskurs 2 (valbar)

Examinator:

Professor  Malin Celander


Kursutvärdering:

http://document.chalmers.se/doc/3d50877a-bff2-4227-b173-59af323288a4


Behörighet:

För kurser inom Chalmers utbildningsprogram gäller samma behörighetskrav som till de(t) program kursen ingår i.

Kursspecifika förkunskaper

Undergraduate profile: Major in Bio Engineering, Chemical engineering, Chemistry or Biology
Prerequisites: Chemistry and biochemistry, Cell and molecular biology

Syfte

The aim of the course is to provide an in-depth knowledge in the areas of general, biochemical and molecular toxicology as applied to eukaryotes and vertebrates in particular, emphasizing mechanisms of action of toxic substances including pharmaceuticals, as well as environmental effects. In addition, the course gives a more general overview of other areas in biochemical toxicology. The course is suitable for students with a specialization towards the pharmaceutical and food industry, environment-related industry and the public sector.

Lärandemål (efter fullgjord kurs ska studenten kunna)

  • Know how environmental pollutants/substances/pharmaceuticals are activated metabolically (cytochrome P450), as well as which elimination systems that exist and which specific enzymes that are involved and their biochemical and regulatory properties.
  • Be familiar with genotoxicity, and techniques and test methods for quantitating genotoxicity, using eukaryotic cells in culture.
  • Know the role of free radicals and radical-generating toxic substances, as well as their roles in toxicity and disease.
  • Be familiar with organ-specific toxicity, especially neurotoxicity.
  • Know about mechanisms of cellular aging processes, carcinogenesis and factors influencing toxicity.
  • Be knowledgeable about basic pharmacokinetics and risk evaluation of chemicals.
  • Be able to use molecular detoxification systems for the detection and assessment of environmental pollutants.

Innehåll

The course describes effects caused by industrial and other human activities on the different life forms in the environment, primarily mammals including human, and gives insights into cellular and molecular aspects of the effects of environmental pollutants in other vertebrates such as fish. The course provides insight in absorption, metabolism and excretion of toxic substances including pharmaceuticals as well as other environmental pollutants. The course provides insight in the molecular mechanisms of action of anthropogenic chemical, as well as the basic molecular biological response mechanisms following an exposure to toxic substances in animals.

The course contains the following parts:
Function and regulation of phase I (cytochrome P450) and phase II (conjugation) enzymes in vertebrates
Induction of cytochrome P450 on mRNA level in cultured cells
Cytochrome P450-mediated hydroxylation activities in cultured cells
Cytochrome P450-dependent drug interactions
Free radicals, their generation and inactivation
Genotoxicity
Different stages of carcinogenesis
Cellular aging processes at the biochemical level
Neurotoxicity
Other types of organ-specific toxicology
Nanoparticals and their toxicity
Pharmacokinetics
Risk-evaluation
Biomonitoring in the aquatic environment
Litterature assignment

Organisation

The course includes about 20 lectures (each 2x45 min) given predominantly by external research specialists from university and industry. There are some topics such as the cytochrome P450 system, oxidative stress, carcinogenesis and neurotoxicity that covered by series, of about 4-8 lectures each, to provide a more in-debt knowledge in biochemical toxicity. Other topics are presented on a more general basis as a ¿smorgasbord¿, of about 2 lectures each, to provide an introduction to different areas of biochemical toxicology both from a human and from an environmental point of view.
The laboratory exercises focus on cellular techniques and regulation of detoxification systems which involves cultures of vertebrate cell-lines, exposure to chemicals in vitro and analysis of detoxification mechanisms on mRNA level using realtime quantitative PCR and enzyme kinetics using fluorescent probes.
The literature assignment involves selection of a topic in biochemical toxicology, literature-search for recent original research publications, writing an abstract, preparing slides followed by an oral presentation.

Litteratur

Research papers
Lecture handouts
Lab methods
Recommended reference literature:
Ernest Hodgson and Robert C. Smart: Introduction to Biochemical Toxicology, Fourth Edition, A John Wiley and sons inc., publication 2008.

Examination

The examination is based on a written exam, grades TH, and approved laboratory exercises.


Sidansvarig Publicerad: må 13 jul 2020.