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Syllabus for

Academic year
KMB050 - Membrane protein technology
 
Owner: TKBIA
5,0 Credits (ECTS 7,5)
Grading: TH - Five, Four, Three, Not passed
Level: A
Department: 21 - CHEMISTRY AND CHEMICAL ENGINEERING


Teaching language: English

Course module   Credit distribution   Examination dates
Sp1 Sp2 Sp3 Sp4 No Sp
0103 Project 5,0 c Grading: TH   5,0 c    

In programs

TKMTA CHEMICAL ENGINEERING, Year 4 (elective)
TKBIA BIOENGINEERING, Year 4 (elective)
TKMTL CHEMICAL ENGINEERING(FOR ENGINEERS WITH U.C.IN CHEMICAL ENG), Year 2 (elective)

Examiner:

Professor  Richard Neutze



Eligibility:

For single subject courses within Chalmers programmes the same eligibility requirements apply, as to the programme(s) that the course is part of.

Course specific prerequisites

Anyone at fourth year within the Bt or K programmes at Chalmers will automatically be accepted. Students at fourth year in other programmes or at Gothenburg University may also be accepted but must contact Arjan Snijder in advance. This course has a maximum of 16 places.

Aim

Students will gain a state-of-the-art knowledge on techniques and methods used in membrane protein research and membrane protein structural biology in particlar.

Goal

See Aim

Content

Membrane proteins facilitate a wide variety of crucial tasks within the cell. Much effort is invested into unravelling their structure and function, thereby understanding fundamental cellular processes. Moreover, membrane proteins form an important class of drug-development targets and are of considerable industrial interest. Membrane protein research has certain peculiarities and pitfalls that require particular knowledge and awareness. This course is designed to teach modern biochemical and biophysical methods focused on membrane proteins.
In the course, membrane proteins will be produced, membrane fractions isolated, membrane solubilization will be addressed and protein purification protocols will be investigated. The purified protein will be characterized using different techniques. In particular the aggregation state of the membrane proteins will be analyzed and different membrane protein crystallization methods will be applied. Detergent and lipid properties will be discussed and analyzed.

Organisation

The course is organized as mini-projects with 12 seminars supplying theoretical background. Mini-projects will be based on projects in the lab and the results are aimed to contribute to the on-going research. Depending on the number of participants, individuals or couples of students will work on a selected project for which they will complete 80 hours of lab work on a full time basis.
It is recommended that students also interested in a deeper theoretical understanding of membrane protein structure & function take in parallel the course KMB035 Membrane transport and bioenergetics".

This course is optimized to be followed together with course KMB035, Membrane transport and bioenergetics .

Literature

The course literature will comprise of selected articles, seminar notes and lab-instructions. "Membrane protein purification and crystallization" by Hunte, von Jagow and Schägger 2003 2nd edition is advised but is not obligatory.

Examination

Students will be graded on the basis of their participation during the course, an oral presentation, and on the basis of the presentation of their results in a report written in English.


Page manager Published: Thu 03 Nov 2022.