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Syllabus for

Academic year
KBB095 - Protein engineering
 
Owner: TKBIA
10,0 Credits (ECTS 15)
Grading: TH - Five, Four, Three, Not passed
Level: A
Department: 21 - CHEMISTRY AND CHEMICAL ENGINEERING


Teaching language: Swedish

Course module   Credit distribution   Examination dates
Sp1 Sp2 Sp3 Sp4 No Sp
0199 Examination 10,0 c Grading: TH   10,0 c   Contact examiner

In programs

TKBIA BIOENGINEERING, Year 4 (elective)

Examiner:

Professor  Jan Rydström



Eligibility:

For single subject courses within Chalmers programmes the same eligibility requirements apply, as to the programme(s) that the course is part of.

Aim

The course is designed to show how modern mole-cular and biochemical methods can be combined and used to specifically predict and test structure-function relations in proteins, a strategy commonly used in biomedical research and the biotechnology industry. This is achieved by selecting two model proteins, preferrably membrane proteins, presently bacterial cytochrome oxidase and transhydro-genase, as test proteins. A particular structure-function is addressed with either of the proteins, a mutagenesis protocol is designed, the mutant gene is sequenced, the mutant gene is expressed, and the recombinant protein is purified and characte-rized biochemically. All steps are carried out by the student, who therefore will learn and practice a number of useful methods in protein chemistry, and interpret mutagenesis information in terms of functional and structural consequences. The course includes a series of lectures in modern biotechnology given mostly by invited experts from e.g. the pharmaceutical industry, a literature task, presented in a manscript format as well as orally, and a study visit to a biomedical research institute or industry.

Literature

J.L. Cleland and C.S. Craik: Protein Engineering (Wiley -Liss, Inc., 1996).

Examination

Written exam.


Page manager Published: Thu 03 Nov 2022.